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. 2018 Oct 31;8:16103. doi: 10.1038/s41598-018-34532-2

Figure 2.

Figure 2

The s38-protein lack essentially affects the length and morphology of dorsal appendages in Drosophila eggs. (A,B) The morphology of control (c355-GAL4/+) laid eggs is characterized by two prominent dorsal appendages, as illustrated through a (A) light microscope and (B) stereomicroscope. (C,D) Characteristic s38-targeted (c355 > s38_RNAi) laid eggs with very short and thin dorsal appendages, and a glassy external surface (D). (E,F) Representative s38-downregulated laid eggs with comparatively longer but still dysmorphic dorsal appendages. Number of eggs examined: control = 291, s38-downregulated = 713. (G) s38 transcript-level quantification of control (c355-GAL4/+) and s38-targeted (c355 > s38_RNAi) follicles carrying either short or medium/long in length dorsal appendages, via employment of an RT-PCR protocol. Actin served as gene of reference. DA: Dorsal Appendage(s). Scale Bars: 50 μm.