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. 2018 Oct 31;3:53. doi: 10.1038/s41541-018-0090-4

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — Analysis of recombinant proteins from glycoprotein expression strains. a-c Immunoblot analysis of decreasing concentrations of recombinant glycoproteins from galE⁺ and galE^- E. coli strains. Fluorescent intensity of the signal from the glycosylated protein bands (green channel) was measured by densitometry using the LI-COR odyssey fluorescent imaging system and are displayed as mean ± SEM from three replicate experiments. Red channel: mouse anti-His IgG; Green channel: rabbit anti-Type 4 antiserum. d-h Anti-capsular polysaccharide antibody levels in antiserum measured from mice (n = 8) vaccinated with recombinant glycoproteins (closed circles) or cognate unglycosylated antigens (open squares). Antiserum from Prevnar-13 (closed circles) and PBS vaccinated (open squares) animals were included as controls. Results displayed as mean ± SEM from technical replicates