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. 2018 Oct 31;3:53. doi: 10.1038/s41541-018-0090-4

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — Effect of antiserum samples on interaction of S. pneumoniae with human neutrophils. a Examples of flow cytometry histograms for fresh human neutrophils incubated with FAM-SE labelled TIGR4 when opsonised in 20% antiserum from glycosylated (red shading) or unglycosylated (grey shading) vaccine groups and 5% baby rabbit complement. PBS vaccinated serum (dashed line) was included as a control. b Percent association of fresh human neutrophils with TIGR4 when opsonised in 20% antiserum (n = 8) from glycosylated (closed circles) or unglycosylated (open squares) vaccine groups and 5% baby rabbit complement. Antiserum from Prevnar-13 (closed circles) and PBS vaccinated (open squares) animals were included as controls. *p < 0.05 Kruskal–Wallis with Dunn’s post-test (vs PBS). c-e Percent association of fresh human neutrophils with non-type 4 pneumococci when opsonised in 20% antiserum (n = 6) from glycosylated (closed circles) or unglycosylated (open squares) vaccine groups and 5% baby rabbit complement. Antiserum from Prevnar-13 (closed circles) and PBS vaccinated (open squares) animals were included as controls