Fig. 6.

Vaccination with recombinant glycoconjugates provides protection against several pneumococcal disease aetiologies. Mice were vaccinated with Combo(Sp4), Combo, Prevnar-13, or PBS+ adjuvant as outlined in the methods. a Mice challenged intranasally with 1 × 10⁷ CFU of TIGR4 were culled seven days post-infection and bacterial loads were assessed by nasal washing. b, c Mice were infected intranasally with 1 × 10⁷ CFU of TIGR4 b or 2 × 10⁶ CFU of WCH43 c and culled after 24 or 48 h respectively. Bacterial burdens in the blood and organ homogenates were determined by serial dilution and plating. *p < 0.05 Kruskal–Wallis with Dunn’s post-test (vs PBS)