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. 2018 Oct 31;9(11):1107. doi: 10.1038/s41419-018-1153-1

Fig. 4. MiR-139-3p directly targets ELK1 in MC3T3-E1 cells, and ELK1 is sensitive to simulated microgravity.

Fig. 4

a Schematic representation of the luciferase reporter containing ELK1 3′UTR WT or ELK1 3′UTR MUT. b The luciferase activity of the ELK1 WT and ELK1 MUT reporter in 293T cells treated with mimic-139, inhibitor-139 or the corresponding controls for 24 h (N = 3). c mRNA expression of ELK1 in osteoblasts treated with mimic-139, inhibitor-139 or the corresponding controls for 48 h (N = 3). d Protein levels of ELK1 and pELK1 in osteoblasts (N = 3). e Immunostaining analysis of the expression levels of ELK1 in osteoblasts (N = 3). Scale bar, 50 µm. f mRNA expression of ELK1 in osteoblasts under clinorotation conditions for 48 h (N = 3). g Protein levels of ELK1 in osteoblasts treated with inhibitor-139 or the corresponding control under clinorotation conditions for 48 h (N = 3). *P < 0.05, **P < 0.01 vs. control