Table 3.
Annotated genes encoding lignocellulose-degrading enzymes in B. velezensis ZY-1-1
| Gene of B. velezensis ZY-1-1 | Reference gene | BLASTp identity (%) | BLASTp coverage (%) | |||
|---|---|---|---|---|---|---|
| Annotation | Accession no. (NCBI) | Accession no. (UniProtKB) | Species | Annotated Gene | Reference Gene | |
| Hemicellulose-related | ||||||
| Endo-1,4-β-xylanase | AVX15758.1 | P18429 | B. subtilis 168 | 95.00 | 100.00 | 100.00 |
| Glucuronoxylanase | AVX17235.1 | Q45070 | B. subtilis 168 | 90.00 | 100.00 | 100.00 |
| 1,4-β-Xylosidase | AVX17306.1 | P94489 | B. subtilis 168 | 94.90 | 100.00 | 88.37 |
| Arabinosidase | AVX16491.1 | P94531 | B. subtilis 168 | 87.10 | 99.40 | 99.20 |
| Arabinosidase | AVX16510.1 | P94552 | B. subtilis 168 | 80.73 | 99.40 | 99.60 |
| Arabinoxylan arabinofuranohydrolase | AVX17234.1 | Q45071 | B. subtilis 168 | 91.56 | 100.00 | 87.72 |
| β-Mannanase | AVX15568.1 | O05512 | B. subtilis 168 | 74.31 | 100.00 | 100.00 |
| Acetylxylan esterase | AVX18602.1 | P94388 | B. subtilis 168 | 83.96 | 100.00 | 100.00 |
| Endo-1,5-α-l-arabinanase | AVX16483.1 | Q93HT9 | Geobacillus thermodenitrificans | 51.00 | 89.41 | 94.25 |
| Galactanase | AVX17829.1 | Q65CX5 | B. licheniformis ATCC 14580 | 30.48 | 79.19 | 70.52 |
| 6-Phospho-β-galactosidase | AVX17823.1 | C7N8L9 | Leptotrichia buccalis ATCC 14201 | 61.00 | 99.36 | 99.36 |
| Cellulose-related | ||||||
| Endo-1,4-β-glucanase | AVX17239.1 | P07983 | B. subtilis DLG | 96.79 | 100.00 | 100.00 |
| β-Glucanase/Endo-β-1,3 − 1,4 glucanase | AVX15545.1 | P07980 | B. amyloliquefaciens | 92.47 | 98.35 | 100.00 |
| β-Glucosidase | AVX18716.1 | Q7WUL3 | Cellulomonas fimi | 29.12 | 65.78 | 68.44 |
| 6-Phospho-β-glucosidase | AVX15571.1 | O05508 | B. subtilis 168 | 81.96 | 98.71 | 98.92 |
| 6-Phospho-β-glucosidase | AVX15585.1 | P46320 | B. subtilis 168 | 94.57 | 100.00 | 100.00 |
| 6-Phospho-β-glucosidase | AVX16352.1 | P46320 | B. subtilis 168 | 29.50 | 99.31 | 97.51 |
| Aryl-phospho-β-d-glucosidase | AVX17112.1 | P42973 | B. subtilis 168 | 88.66 | 99.17 | 99.37 |
| Oligo-1,6-glucosidase | AVX16273.1 | P29093 | Bacillus sp. F5 | 47.56 | 90.40 | 99.61 |
| Oligo-1,6-glucosidase | AVX18210.1 | O06994 | B. subtilis 168 | 50.27 | 98.04 | 99.11 |
| Oligo-1,6-glucosidase | AVX18633.1 | O06994 | B. subtilis 168 | 49.19 | 98.57 | 98.75 |
| 6-Phospho-α-D-glucosidase | AVX18175.1 | P54716 | B. subtilis 168 | 92.43 | 100.00 | 100.00 |
| Lignin-related | ||||||
| Laccase | AVX18327.1 | D4GPK6 | Haloferax volcanii ATCC 29605 | 38.92 | 98.83 | 85.32 |
| Deferrochelatase | AVX15607.1 | Q8XAS4 | Escherichia coli O157:H7 | 39.04 | 84.93 | 86.76 |
The references which confirmed the specific enzymatic activities of the reference genes are listed in Table S2