Figure 2.

Deficiency of hepatic BMAL1 attenuates hyperglycemia in HFD-fed mice. (A) Immunoblot showing phosphorylation levels of BMAL1 in RD- or HFD-fed mice. (B) Immunoblot of BMAL1 recovered from immunoprecipitations of HDAC5 from liver samples in RD- or HFD-fed mice. (C) Weight gain in BMAL1 LKO and control littermates fed on HFD (n = 6). (D) Blood glucose levels under ad lib and fasted conditions in BMAL1 LKO and control littermates maintained on HFD (n = 6). (E) Pyruvate tolerance test of BMAL1 LKO and control littermates maintained on HFD (n = 6). (F) Effect of fasting on mRNA amounts for hepatic gluconeogenic genes in liver of BMAL1 LKO and control littermates maintained on HFD (n = 6). (G) Schematic of proposed mechanism. All data are presented as mean ± s.e.m. *P < 0.05