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. 2018 Jan 30;9(11):930–944. doi: 10.1007/s13238-018-0507-x

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© The Author(s) 2018

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

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RBM24 blocks 80S ribosome assembly on HCV IRES. Biotin-labeled JFH1 nt 1–360 together with rhRBM24 or BSA were incubated in RRL at 30°C for 15 min. The ribosome complexes were separated by sucrose gradient ultracentrifugation. The distribution of biotin-RNA was detected by dot-blot assay with streptavidin-HRP and analyzed by densitometry (fractions 5–21 were shown)