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. 2018 Jan 30;9(11):930–944. doi: 10.1007/s13238-018-0507-x

Figure 6.

Figure 6

RBM24 enhances the interaction between the HCV 5′- and 3′-UTRs. (A) Interaction between the 5′-UTR (J1–360 or J1–149) and 3′-UTR (J9440–9678) was analyzed in the presence or absence of rhRBM24, as described in the experimental procedures. Precipitated RNAs were analyzed on a 6% urea-PAGE gel and by autoradiography. (B) The effects of cold RNA competitors (J1–360 and J9440–9678) on the interaction between HCV J1–149 and J 9440–9678 were analyzed. (C) The 293T cells were co-transfected with pFlag-RBM24 and pHA-RBM24. Cell lysates were immunoprecipitated with either an anti-Flag mouse monoclonal antibody (Flag), an anti-HA mouse monoclonal antibody (HA) or a nonspecific mouse control antibody (IgG). Precipitated proteins were detected with an anti-HA rabbit monoclonal antibody (HA) or an anti-DYKDDDDK rabbit polyclonal antibody (Flag) correspondingly