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Primer sequences for real-time PCR assay
| Gene | Forward primer (5′–3′) | Reverse primer (5′–3′) |
|---|---|---|
|
| ||
| TGF-β1 | ATTCCTGGCGTTACCTTGG | AGCCCTGTATTCCGTCTCCT |
| TNF-α | TCAGTTCCATGGCCCAGAC | GTTGTCTTTGAGATCCATGCCATT |
| Cox-2 | GGATCATCAACACTGCCTCA | ATGGTGGCTGTCTTGGTAGG |
| IL-1β | TGATGACGACCTGCTAGTGTG | TCCATTGAGGTGGAGAGCTT |
| IL-6 | AGAGACTTCCAGCCAGTTGC | ACAGTGCATCATCGCTGTTC |
| GAPDH | TGGATTTGGACGCATTGGTC | TTTGCACTGGTACGTGTTGAT |
Abbreviations: GAPDH, glyceraldehyde phosphate dehydrogenase; TNF-α, tumor necrosis factor alpha.
