Fig. 2.

Time course (a) and concentration dependence (b) of pregabalin uptake by hCMEC/D3 cells. (a) Pregabalin uptake (10 μM) by hCMEC/D3 cells was examined at 37°C in the presence (●) or absence (○) of sodium. (b) Pregabalin uptake by hCMEC/D3 cells was examined at 37°C for 10 min. Data were subjected to Michaelis–Menten (main) and Eadie–Scatchard (inset) analyses. In the Michaelis–Menten plot, the solid, dotted, and dashed lines represent saturable, nonsaturable, and overall pregabalin uptake, respectively. In the Eadie–Scatchard plot, the solid line represents the overall pregabalin uptake. Each point represents the mean ± SEM (n = 3–4).