Table II.

Inhibitory effect of pregabalin on LAT1 and LAT2-mediated amino acid uptake

	[14C]L-Leucine uptake (μL/mg-protein)		Transporter-mediated [14C]L-leucine uptake (μL/mg-protein)
	Control	+ 1 mM pregabalin	Control	+ 1 mM pregabalin
Mock	247 ± 5	66.4 ± 4.5*	–	–
LAT1	485 ± 29	103 ± 7*	237 ± 29 [100]	36.8 ± 8.5* [15.5]
LAT2	345 ± 13	192 ± 7*	97.8 ± 14 [100]	126 ± 6 [129]

LAT1-, LAT2-overexpressing, and mock cells were incubated with [¹⁴C]L-leucine (0.4 μM) in the presence or absence (Control) of 1 mM pregabalin at 37°C for 2 min. Transporter-mediated uptake was calculated by subtracting the uptake by mock cells from that by LAT-overexpressing cells. Values in square brackets represent percentage of control uptake. Each value represents the mean ± SEM (n = 3). *p < 0.05, significantly different from control