Table III.

Inhibitory effect of various compounds on pregabalin uptake by hCMEC/D3 cells

	Compound	Uptake (% of Control)
	Control	100 ± 5
	Na+ − free	102 ± 7
1 mM	BCH	17.0 ± 3.0*
1 mM	L-Leu	16.7 ± 2.7*
1 mM	L-His	20.6 ± 1.0*
1 mM	L-Phe	ULQ (< 6.15)
1 mM	L-Trp	ULQ (< 5.66)
10 μM	JPH203	ULQ (< 6.53)
1 mM	L-Glu	117 ± 6
1 mM	L-Arg	101 ± 2
10 mM	MeAIB	80.8 ± 4.0*
1 mM	TEA	89.8 ± 4.3
1 mM	PAH	115 ± 7

hCMEC/D3 cells were incubated with pregabalin (10 μM) in the presence or absence (Control) of various compounds at 37°C for 10 min. Each value represents the mean ± SEM (n = 4–12). The uptake in the presence of 1 mM L-Phe, 1 mM L-Trp, or 10 μM JPH203 was below lower limit of quantification. ULQ means under the limit of quantification. The value in parentheses following ‘ULQ’ represents the value of the quantification limit. *p < 0.05, significantly different from control