Table 1.
Primers used in Q-PCR amplification
| Gene | Name | Sequence (5′→3′) | Tm (°C) | Amplikon length (bp) |
|---|---|---|---|---|
| PMM1 (ref.) | hPMMaF | CGCCTTCCTGCAGAAGCTAC | 59 | 176 |
| hPMMaR | TCTGCTTGGAGAGCAGTCGTC | 59 | ||
| GUSB (ref.) | hGUSBaF | CAGGGTTTCACCAGGATCCAC | 60 | 176 |
| hGUSBaR | TTTATTCCCCAGCACTCTCGTC | 59 | ||
| ACTN1 | hACTN1aF | AGAAATCGTGGATGGGAATG | 60 | 190 |
| hACTN1aR | GCCATCCTTCCAGCTTATG | 60 | ||
| COL17A1 | hC17A1aF | GTACATGCAGAGTGACAGTATTA | 60 | 190 |
| hACTN1aR | AGAGATGGAGGACGAGAAC | 60 | ||
| FOXO1 | hFOXO1aF | TGACTTGGATGGCATGTTC | 60 | 190 |
| hFOXO1aR | GTGTAACCTGCTCACTAACC | 60 | ||
| ITGB4 | hITGB4aF | CTGGAAGGTCACCAACAAC | 60 | 192 |
| hITGB4aR | TAGACCTCGTTCAGGTTCTC | 60 | ||
| SOD2 | hSOD2aF | TCCAGGCAGAAGCACAG | 61 | 140 |
| hSOD2aR | TTCTCCTCGGTGACGTTC | 61 |