TABLE 2.
Strains and plasmids for bacitracin MIC measurement
| Strain or plasmid | Description | Reference |
|---|---|---|
| Strains | ||
| E. coli | ||
| SM10 | thi thr leu tonA lacY supE recA RP4-2-Tc::Mu (Km) | 64 |
| BYF684 | SM10 harboring pYF684 | This study |
| S17.1 | thi pro hsdR RP4-2-Tc::Mu Km::Tn7 (Tp Sm) | 64 |
| BYF701 | S17.1 harboring pRP1099 | This study |
| BYF794 | S17.1 harboring pYF794 | This study |
| BYF796 | S17.1 harboring pYF796 | This study |
| BYF797 | S17.1 harboring pYF797 | This study |
| BYF798 | S17.1 harboring pYF798 | This study |
| BYF799 | S17.1 harboring pYF799 | This study |
| BYF800 | S17.1 harboring pYF800 | This study |
| BYF801 | S17.1 harboring pYF801 | This study |
| BYF802 | S17.1 harboring pYF802 | This study |
| BYF803 | S17.1 harboring pYF803 | This study |
| B. anthracis | ||
| 34F2 | B. anthracis vaccine strain | 62 |
| BYF10028 | 34F2 with the region between rrnG and rrnH deleted (DelGH) | This study |
| BYF10031 | BYF10028 harboring pYF794 | This study |
| BYF10033 | BYF10028 harboring pYF798 | This study |
| BYF10034 | BYF10028 harboring pYF799 | This study |
| BYF10035 | BYF10028 harboring pYF800 | This study |
| BYF10036 | BYF10028 harboring pYF801 | This study |
| BYF10037 | BYF10028 harboring pYF796 | This study |
| BYF10038 | BYF10028 harboring pYF797 | This study |
| BYF10039 | BYF10028 harboring pYF802 | This study |
| BYF10040 | BYF10028 harboring pYF803 | This study |
| Plasmids | ||
| pRP1028 | Vector for introduction of I-SceI recognition site | 61 |
| pYF684 | pRP1028 cloned with sequence around bcrB of 34F2 | This study |
| pRP1099 | Vector for introduction of the gene encoding I-SceI | 61 |
| pYF794 | pRP1099 without I-SceI and AmCyan | This study |
| pYF796 | pYF794 with cloned uppP, bcrB, and bcrA of 34F2 | This study |
| pYF797 | pYF794 with cloned bcrB and bcrA of 34F2 | This study |
| pYF798 | pYF794 with cloned bcrA of 34F2 | This study |
| pYF799 | pYF794 with cloned bacS and bacR of 34F2 | This study |
| pYF800 | pYF794 with cloned bacR of 34F2 | This study |
| pYF801 | pYF794 with cloned uppP of 34F2 | This study |
| pYF802 | pYF794 with cloned bcrB of 34F2 | This study |
| pYF803 | pYF794 with cloned bacS of 34F2 | This study |