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. 2018 Jul 28;97(12):2299–2308. doi: 10.1007/s00277-018-3451-1

Fig. 1.

Fig. 1

Estimation of limit of detection (LoD) for qPCR and ddPCR methods. For qPCR, fractions of positive reads were fitted to sigmoidal curve and the LoD was estimated at 95% confidence level. For ddPCR, LoD calculation was based on limit of blank (LoB) value and standard deviation (SD) of measurements