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. 2017 Sep 7;25(32):31848–31862. doi: 10.1007/s11356-017-0048-5

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© The Author(s) 2017

Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

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Fig. 8 — Agarose gel (1.0%) electrophoresis of some PCR products of DNA samples extracted from enrichment culture obtained with universal and specific primers: lanes M—molecular weight markers; lanes 1, 3, and 5—negative controls; lane 2—DNA sample (A-3) amplified with 16Sp RNA gene; lane 4—DNA sample (A-3) not amplified with alkane hydroxylase genes (alkB-F and alkB-R); lane 6—DNA sample (A-3) amplified with cytochrome P450 hydroxylase genes (P450F and P450R)