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. 2018 Mar 9;132(6):1555–1573. doi: 10.1007/s00414-018-1817-5

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© The Author(s) 2018

Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

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Fig. 2 — Annealing/extension temperature analysis. 1.0 ng of Control DNA 007 was amplified with GlobalFiler™ Kit for 29 cycles over indicated range of annealing/extension temperatures. Markers D10 and D19 were the most profoundly impacted loci. Reaction products were analyzed on an Applied Biosystems 3500xL Genetic Analyzer (Y-axis scale 0 to 16,000 RFU)