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Omitting the final extension step results in shoulders on main allele peaks due to incomplete A nucleotide addition. Final extension step times of 0 min (top three panels) and 10 min (bottom three panels) were tested. Examples shown are the smaller amplicons of 6-FAM™, NED™, and SID™ dye channel data from a 3500xL Genetic Analyzer using the GlobalFiler™ Kit
