Table 3.
Mock casework samples. Alleles recovered from mock samples (described in Methods Section) in GlobalFiler™ (GF) and Identifiler™ Plus (IDP) runs were tabulated. Percentage of alleles recovered out of total possible (when known) for each kit are also presented. Quantification values for the extracted samples are also presented, along with DNA input amounts for the STR amplification reactions
| Sample | Sample DNA | DNA input (ng/reaction) | Alleleles called | % of tot. alleles | |||
|---|---|---|---|---|---|---|---|
| (ng/μL) | GF | IDP | GF | IDP | GF | IDP | |
| Soda Can Swab 1 | 0.081 | 1.00 | 0.81 | 40 | 26 | 100% | 100% |
| Soda Can Swab 2 | 0.185 | 1.00 | 1.00 | 40 | 26 | 100% | 100% |
| Soda Can Swab 3 | 0.079 | 1.00 | 0.79 | 40 | 26 | 100% | 100% |
| Cig Butt 1 | 0.012 | 0.19 | 0.12 | 18 | 11 | n/a | n/a |
| Cig Butt 2 | 0.983 | 1.00 | 1.00 | 40 | 28 | n/a | n/a |
| Cig Butt 3 | 0.830 | 1.00 | 1.00 | 40 | 28 | n/a | n/a |
| Blood on Soil Day0 | 3.419 | 1.00 | 1.00 | 40 | 27 | 100% | 100% |
| Blood on Soil Day1 | 0.757 | 1.00 | 1.00 | 40 | 27 | 100% | 100% |
| Blood on Soil Day2 | 0.004 | 0.06 | 0.04 | 24 | 18 | 60% | 67% |
| Blood on Soil Day3 | 0.001 | 0.01 | 0.01 | 0 | 0 | 0% | 0% |
| Blood on Wood | 0.014 | 0.21 | 0.14 | 41 | 27 | 100% | 100% |
| Blood on Metal | 0.025 | 0.38 | 0.25 | 41 | 27 | 100% | 100% |
| Blood on Metal (Flocked Swab) | 0.018 | 0.28 | 0.18 | 41 | 27 | 100% | 100% |
| Semen on Cloth | 2.783 | 1.00 | 1.00 | 41 | 27 | 100% | 100% |
| Semen on Cloth with UV | 0.214 | 1.00 | 1.00 | 41 | 27 | 100% | 100% |
| Average per sample | 35.0 | 23.5 | |||||