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. 2018 Jun 16;127(4):437–459. doi: 10.1007/s00412-018-0674-9

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© The Author(s) 2018

Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

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Fig. 10 — Delayed histone H1t expression in Tex19.1^−/− spermatocytes depends on Spo11. a Immunostaining for histone H1t (green) in Spo11^−/− Tex19.1^+/± single knockout and Spo11^−/− Tex19.1^−/− double knockout zygotene-like spermatocyte chromosome spreads. Example histone H1t-positive and H1t-negative images are shown. SYCP3 (red) marks the lateral element of the synaptonemal complex respectively. Scale bars 10 μm. b Quantification of the proportions of zygotene-like nuclei that are H1t-positive; 49.5% of Spo11^−/− Tex19.1^+/± and 50.5% of Spo11^−/− Tex19.1^−/− zygotene-like nuclei obtained from three animals of each genotype were H1t-positive (n = 97, 99; χ² test, ns indicates no significant difference, p > 0.05)