Fig. 1.

PRSS55 is a GPI-anchored membrane protein specifically expressed in adult mouse testis. a Tissue expression profile of Prss55 mRNA in adult mice is shown by semi-quantitative and real-time quantitative RT-PCR. Actb serves as an internal control. Bar chart shows mean ± SE values from three replicates. b Prss55 mRNA relative to Actb levels in the testis and epididymis of the mice at the indicated age. Bar chart shows mean ± SE values from three replicates. c PRSS55 protein levels in the testis and epididymis of the mice at the indicated age, and GAPDH as a loading control are shown. d The protein levels in cytoplasmic (C), membrane (M) and nuclear (N) subfractions of adult mouse testicular cells were analyzed by Western blot. GAPDH, LAMIN A/C and NaKATPASE represent cytoplasmic, nuclear and membrane fractions, respectively. e The intact testicular cells from adult mice were treated with the indicated concentrations of PI-PLC, and the supernatants were collected for Western blot analysis. uPAR was detected as a positive control for GPI-anchored protein