Table 2.
Comparative proteomics of ciliary proteins in wild-type and FAP43 and FAP44 mutant cells
| Proteins | Number of unique peptides | |||
|---|---|---|---|---|
| WT | FAP43-KO | FAP43coDel | FAP44coDel | |
| Atu1 α-tubulin | 2739/53 | 1924/52 | 2890/53 | 2646/50 |
| Btu1 β-tubulin | 3058/56 | 2423/56 | 2949/52 | 3072/51 |
| ODA Dyh3 | 1616/425 | 1055/426 | 1411/440 | 877/261 |
| ODA Dyh4 | 1702/448 | 1127/424 | 1395/445 | 981/284 |
| ODA Dyh5 | 1164/345 | 906/355 | 990/355 | 701/225 |
| IDA I1a Dyh6 | 440/234 | 371/236 | 377/222 | 98/59 |
| IDA I1b Dyh7 | 387/220 | 356/252 | 359/218 | 86/61 |
| Fap43 | 100/69 | 0 | 0 | 0 |
| Fap44 | 88/64 | 0 | 0 | 0 |
| Fap57A | 43/36 | 43/36 | 40/35 | 21/20 |
| Fap57B | 19/17 | 29/26 | 15/14 | 9/9 |
| Fap57C | 19/18 | 12/12 | 8/8 | 5/5 |
Mass spectrometry analysis of the axonemal proteins from Tetrahymena wild-type, FAP43 knockout (FAP43-KO and FAP43coDel) and FAP44coDel mutant cells. The table shows the numbers (X/Y) of all the identified peptides (X) and identified unique peptides (Y). Note that Fap43p and Fap44p are missing in mutant but not in wild-type axonemes (all identified proteins are listed in Table S6)