Fig. 1.

eIF2A directly interacts with eIF5B. GST pull-down experiments were performed with recombinant His–eIF2A, GST–eIF5B (587–1220), GST–PABP, and GST, which were purified from E. coli extracts. The GST pull-down samples (lanes 5–7) were resolved by SDS-PAGE and visualized by western blotting with anti-His (R&D Systems) for His-tagged eIF2A or anti-GST [32] for GST-fusion proteins. The input proteins (lanes 1–3) correspond to 10% of the proteins used for immunoprecipitation