Fig. 3.

miR-153 suppresses MCF7 proliferation and migration by silencing ANG1. a A schematic illustration of cell treatment, collection and detection. b Downregulation of ANG1 protein levels after ANG1 siRNA (50 nM) treatment for 72 h in MCF7 cells as detected by Western blot. c Knockdown of ANG1 by siRNA decreased cellular viability of MCF7 cells as characterized by the SRB assay. **p < 0.01, t test. d Knockdown of ANG1 with siRNA inhibited the migration of MCF7 cells characterized with the transwell assy. The representative images are shown, and the scale bar is 100 µm. *p < 0.05, **p < 0.01, t test. e Ectopic expression of ANG1 in MCF7 without the miR-153 binding site. f Ectopic expression of ANG1 increased cellular viability of MCF7 cells and rescued the inhibition of miR-153, shown with the SRB assay. *p < 0.05, t test. g Ectopic expression of ANG1 increased the migration of MCF7 cells and rescued the inhibition of miR-153, shown using the transwell assay. Representative images are shown, and the scale bar is 100 µm. **p < 0.01, t test