Fig. 3.

NOS3 3′UTR is a direct target for miR-200b, miR-200c, and miR-429. a Luciferase reporter constructs containing wild-type (Vn) or mutated (Vmut) NOS3 3′UTR sequence were transfected together with miR-200b or scramble control into HEK-293 cells. As a control reporter vector without 3′UTR (Vc) was used. The normalized R.L.U luciferase activities were calculated as a Vn/Vc or Vmut/Vc ratio (n = 2; *p < 0.05 vs. Vn/Vc). Data represent the mean ± SD of three experiments. *p < 0.05 was considered significant. b The schematic presentation of the mutations (bold) introduced into miR-200b TS in NOS3 3′UTR sequence