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. 2018 May 8;21(4):711–724. doi: 10.1007/s10456-018-9620-y

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© The Author(s) 2018

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

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Fig. 4 — miR-200b downregulates eNOS expression in response to hypoxia. HUVECs were transfected with miR-200b mimic, mir-200b antagomiR, or scramble control and cultured in normoxia or hypoxia for 12 h. a NOS3 mRNA levels were quantified by qRT-PCR and normalized to TBP mRNA. Data represent the mean ± SD of two independent experiments. b Corresponding changes in eNOS protein levels were monitored with western blot and normalized to β-Actin and related to the normoxic control. Data represent the mean ± SE of two independent experiments. *p < 0.05 was considered significant