Fig. 3.

Nintedanib affected microvascular barrier integrity and permeability. a 70 kDa RITC-dextran was added to one of the reservoirs and filled the microvasculature in the central chamber. Images represent a segment of the central chamber and are overlays of the red channel and bright field. Time interval between the two images in each case is 1 min. In the 10 nM-treated chip, fluorescent dye leaked from the vasculature into the fibrin gel. The robust integrity of untreated microvessel walls kept the dye inside the vasculature and prevented any obvious leakage into the fibrin gel. Scale bar: 150 µm. b Time lapse images were recorded, and the vascular permeability coefficient was calculated from measurements of the fluorescence intensity across vascular walls over time. At least five areas per chip were analyzed. Treatment started on day 1. n = 6 and p < 0.0056