Fig. 3.

Investigation of the effect of DDAH I on VEGF expression in vitro and in vivo. a VEGF levels in culture medium from cells treated with 2 µg/ml DOX for 6 days. For the last day, cells were also stimulated with cytokines (10 ng/ml TNF-α, 1000 U/ml IFN-γ) and 5 µg/ml LPS. Culture medium was collected from the last 24 h of treatment. b VEGF expression in tumour homogenates. C6 DDAH tumours (group A) derived from animals provided with drinking water containing 0.2 mg/ml DOX in 5% (w/v) sucrose or 5% (w/v) sucrose alone (n = 6 per group). Mice with D27 tumours were given drinking water alone (n = 4). Results are mean + 1 SEM of duplicate measurements from three separate in vitro experiments or from all tumours in each group (*P < 0.05, ****P < 0.0001, One-Way ANOVA with Bonferroni’s multiple comparison post-test)