Fig. 4.

Fzd5 knockdown did not affect the canonical Wnt signaling pathway in ECs. a Representative Western blot result of total levels of β-catenin, non-phospho-β-catenin, phospho-β-catenin (ser33/37/thr41), and β-actin loading control, at different time points post-transfection. b Quantified results of β-catenin Western blot. Shown are β-catenin levels relative to β-actin loading control. N = 3, no significance. c Western blot result of total levels of β-catenin and phospho-β-catenin in response to treatment with the phosphatase inhibitor Calyculin A (50 nM) with and without a 30 min pretreatment of the GSK3β inhibitor LiCl (20 mM). d QPCR analysis of the mRNA expression levels of β-catenin target genes Axin2, Cyclin D1 (Ccnd1), and C-myc in different conditions 72 h post-transfection. N = 4, no significance. e Immunofluorescent staining β-catenin (green), F-actin (red), and DAPI (blue) in confluent and sub-confluent f HUVECs after knockdown of Fzd5. N = 3