Table 1.

The thousand grain weight and grain morphometric parameters for CRISPR-Cas9-induced TaGW2 gene mutants (2017 Spring experiment)

Genotype	N	TGW	Area	Width	Length
AABBDD	12	28.79 ± 0.12	12.88 ± 0.04	3.15 ± 0.005	5.54 ± 0.011
aaBBDD	4	30.2 ± 0.22	13.36 ± 0.1	3.22 ± 0.018	5.63 ± 0.019
		4.89%*	3.69%*	2.22%*	1.71%
AAbbDD	6	30.47 ± 0.34	13.49 ± 0.11	3.23 ± 0.01	5.67 ± 0.035
		5.83%*	4.73%*	2.50%*	2.34%
AABBdd	6	30.72 ± 0.56	13.64 ± 0.16	3.24 ± 0.024	5.71 ± 0.033
		6.71%	5.90%*	2.91%*	3.00%*
aaBBdd	3	31.18 ± 0.92	13.33 ± 0.15	3.3 ± 0.046	5.48 ± 0.051
		8.29%*	3.51%	4.75%**	− 1.07%
AAbbdd	4	34.41 ± 0.36	14.39 ± 0.09	3.37 ± 0.014	5.78 ± 0.013
		19.51%****	11.68%****	7.02%****	4.34%**
aabbdd	5	34.14 ± 0.25	14.51 ± 0.04	3.39 ± 0.005	5.84 ± 0.013
		18.56%****	12.63%****	7.57%****	5.52%***
ANOVA (P value)		0.000104	6.282e−05	9.372e−05	0.00347

All data are shown as mean ± standard error. The phenotype data of each genotype were compared to that of genotype AABBDD using the two-tailed Student’s t test; *significant at 0.01 < P < 0.05, **significant at 0.001 < P < 0.01, ***significant at 0.0001 < P < 0.001, ****significant at P < 0.0001. Since there were no phenotypic differences between regenerated plants with genotype AABBDD and wheat cultivar Bobwhite, the data from these plants were combined for analysis