Table 2.

The thousand grain weight and grain morphometric parameters for CRISPR-Cas9-induced TaGW2 gene mutants (2017 Fall experiment)

Genotype	N	TGW (g)	Area (mm2)	Width (mm)	Length (mm)
Bobwhite	11	40.85 ± 0.53	15.97 ± 0.14	3.53 ± 0.018	6.1 ± 0.038
AABBDD	37	39.95 ± 0.16	15.96 ± 0.08	3.54 ± 0.056	6.11 ± 0.029
aaBBDD	26	41.49 ± 0.46	15.99 ± 0.13	3.6 ± 0.017	6.09 ± 0.032
		3.85%*	0.19%	1.69%*	− 0.33%
AAbbDD	53	41.85 ± 0.37	16.25 ± 0.11	3.6 ± 0.013	6.17 ± 0.032
		4.76%**	1.82%	1.69%**	0.98%
AABBdd	28	43.15 ± 0.56	16.69 ± 0.16	3.62 ± 0.017	6.34 ± 0.045
		8.01%****	4.57%***	2.26%**	3.76%****
aabbDD	7	44.58 ± 0.45	16.65 ± 0.16	3.7 ± 0.022	6.04 ± 0.048
		11.59%***	4.32%**	4.52%***	− 1.15%
aaBBdd	16	44.36 ± 0.88	16.69 ± 0.18	3.69 ± 0.022	6.16 ± 0.032
		11.04%****	4.57%***	4.24%****	0.82%
AAbbdd	30	44.79 ± 0.4	16.83 ± 0.12	3.68 ± 0.016	6.21 ± 0.029
		12.12%****	5.45%****	3.95%****	1.64%**
aabbdd	37	46.33 ± 0.38	17.24 ± 0.1	3.74 ± 0.013	6.28 ± 0.026
		15.97%****	8.02%****	5.65%****	2.78%****
ANOVA (P value)		< 2.2e−16	2.531e−15	< 2.2e−16	3.175e−08

All data are shown as mean ± standard error. The phenotype data of each genotype was compared to that of genotype AABBDD using the two-tailed Student’s t test; *significant at 0.01 < P < 0.05, **significant at 0.001 < P < 0.01, ***significant at 0.0001 < P < 0.001, ****significant at P < 0.0001. There were no phenotypic differences between regenerated plants with genotype AABBDD and wheat cultivar Bobwhite