Fig. 6.

Raman mapping of carbon nanomaterials in cells harvested from the peritoneal cavity at 1 day post-injection. Cells were harvested from the peritoneal cavity of mice exposed to l-GO, s-GO and MWCNTs, and seeded on glass coverslips before fixation in 100% methanol cooled at − 20 °C (bright-field images). a Raman spectroscopy showed a strong interaction between these cells and s-GO, as shown by the correlation maps overlaid with the region of interest (ROI). On the other hand, only trace amounts of l-GO were detected in the vicinity of these cells (highlighted with blue arrows), whereas MWCNTs were not detected in association with peritoneal cavity cells. b Selected areas in maps shown in a as highlighted by yellow boxes were magnified for clarity. Scale bars 50 µm. Both GO materials were dispersed in 5% dextrose solution, whereas long MWCNTs were dispersed in 0.5% BSA solution. (Colour figure online)