Fig. 3.

UHPLC SEC chromatograms with the column Acquity UPLC Protein BEH SEC, 4.6 × 150 mm, 125A, 1.7 μm, size range 1 kDa–80 kDa, at a flow rate of 400 μL/min and an injection volume of 5 μL of (A) a protein/amino acid mixture containing standards of HSA (66 kDa)(~ 9.32 μM), Myoglobin (17 kDa) (~ 34.6 μM), glutathione disulfide (GSSG, 0.6 kDa) (~ 375 μM) and methionine (0.1 kDa) (~ 1700 μM) and (B) undiluted FCS. Sulfur was monitored as ³²S¹⁶O at m/z 47.97 with oxygen as reaction gas