Table 2.

Production parameters of C. utilis ATCC 9950 cell biomass, cell wall, β-glucan preparations, and β(1,3)/(1,6)-glucan depending on scale and time of cultivation

	Output (volumetric productivity)				Yield		β(1,3)/(1,6)-Glucan productivity**	Specific rate of β(1,3)/(1,6)-glucan formation**
Type of culture	Cell mass production	Cell wall preparation	β-Glucan preparation	β(1,3)/(1,6)-Glucan **	Cell mass yield	β(1,3)/(1,6)-Glucan yield**
		(gd.w./L)			(gd.w./g glycerol consumed)		(g/L/h)	(g β-glucan/gd.w.biomass/h)
*Flask/72h	25.6 ± 0.8a	6.4 ± 0.6a	3.5 ± 0.9a	2.8	0.383	0.042	0.039	0.0015
Biof/48h	31.3 ± 0.5b	8.1 ± 0.5b	5.3 ± 0.8b	4.5	0.440	0.063	0.094	0.0030
Biof/72h	32.2 ± 1.9b	8.4 ± 0.9b	5.1 ± 0.7b	4.4	0.423	0.058	0.061	0.0019

*Flasks/72h—cultivation in flask during 72 h; Biof/48h and Biof/72h—cultivations in biofermentor adequately 48 and 72 h; ** based on the β(1,3)/(1,6)-glucan concentration in purified β-glucan preparations; a, b, c…—mean values marked with the same letters do not differ significantly, Tukey’s test, α = 0.05. Each value represents the mean and standard deviation from three independent experiments in flask and two independent cultures in biofermentor for each of studied time of cultivation. Replicates were done on different days with different inocula stocks, using the same batch of deproteinated potato juice water as a medium