Fig. 10.

Effect of GPER blockage on aromatase and estrogen receptors localization in mouse testes. Representative microphotographs of testicular sections of control and G-15 (50 μg/kg bw)-treated immature, mature and aged mice. Localization of ERβ (a–f) in testes of control and G-15-treated mice, respectively. Slight decrease in ERβ expression is observed in cytoplasm of immature males when compared to control whose expression is strong (arrows) (a, b). Moderate in control mature and weak in G-15 mature males expression of ERβ is revealed exclusively in cytoplasm of Leydig cells (arrows) (c, d). In control aged males, moderate cytoplasmic expression of ERβ is seen (arrows) (e) but is nuclear in a few Leydig cells of G-15 males (arrows) (f). In negative controls, no positive staining is seen (inserts e). Immunoreaction was performed on testicular serial sections from at least three animals of each experimental group