Fig. 12.

Effect of GPER on sex steroid concentration in mouse testes and secretion by Leydig cells in vitro. Androgens and estrogens concentration in testes of immature, mature and aged mice control and G-15-treated (a, b) and progesterone secretion in MA-10 Leydig cells (c) control and G-15, E2 and ICI-treated alone and in combination. Data are expressed as means ± SD. From each animal, at least three samples were measured. Culture media were measured in triplicate. Asterisks show significant differences in testosterone and estradiol concentrations between control and G-15 (50 μg/kg bw)-treated males and in progesterone secretion between control MA-10 Leydig cells and those treated with G-15 (10 nM), ICI (ICI 182,780; 10 μM), E2 (17β-estradiol; 10 nM) alone and in combination for 24 h. Values are denoted as ∗∗p < 0.01 and ∗∗∗p < 0.001