Fig. 5.

Effect of GPER blockage on Leydig cell ultrastructure. Representative microphotographs of Leydig cells of control and G-15 (50 μg/kg bw)-treated mice. a–e Aged mouse Leydig cells ultrathin sections. Each testicular sample in the epoxy resin block was cut for at least three ultrathin sections that were analyzed. Bars represent 1 μm. Analysis was performed on testicular blocks from at least three animals of each experimental group. Aged Leydig cells exhibit normal morphology. In control aged Leydig cells, normal number and localization of endoplasmic reticulum (er), mitochondria (m) and lipid droplets (ld) are seen (a, b). (c–e) Note, in G-15 aged Leydig cells, the concentric structure of endoplasmic reticulum (er) cisternae (asterisks; c, e) in between normal-looking and normal-distributed mitochondria (c, d). (nu) nucleus