Table 2.
Primary antibodies used for immunocyto-, immunohisto- and immunofluorescence
| Antigen | Host species of primary antibodies | Vendor | Dilution | Host species of secondary antibodies | Vendor |
|---|---|---|---|---|---|
| GPER | Rabbit | Abcam cat.no. ab39742 |
1:50 (ICC) 1:250 (IHC) |
Biotinylated goat anti-rabbit (ICC, IHC) | Vector Laboratories BA-1000 |
| 1:100 (IF) | Alexa Fluor 488 goat anti-rabbit (IF) | Thermo Fisher Scientific A-11008 |
|||
| P450arom | Rabbit | Santa Cruz Biotechnology cat.no. sc-30086 |
1:100 (ICC) 1:500 (IHC) |
Goat anti-rabbit | Vector Laboratories BA-1000 |
| ERα | Rabbit | Abcam cat.no. ab75635 |
1:20 (ICC) 1:100 (IHC) |
Goat anti-rabbit | Vector Laboratories BA-1000 |
| ERβ | Rabbit | Abcam cat.no. ab3576 |
1:20 (ICC) 1:50 (IHC) |
Goat anti-rabbit | Vector Laboratories BA-1000 |
GPER G-coupled membrane estrogen receptor, P450arom cytochrome P450 aromatase, ERα estrogen receptor alpha, ERβ estrogen receptor beta