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. 2018 Oct 31;14(10):e1007724. doi: 10.1371/journal.pgen.1007724

Fig 4. Injection of human TNFα increases sleep in Eiger mutants.

Fig 4

(A,B) Baseline sleep profiles are shown for EGR1 mutants (A) and EGR3 mutants (B) injected with vehicle (PBS) or 1000nM recombinant TNFα. Two-way ANOVA revealed a significant time by drug interaction for the EGR1 mutants (F(1,20) = 91.49, p < 0.0001) and for the EGR3 mutants (F(1,20) = 18.74, p < 0.0001). Tukey Post Hoc analysis was used to identify significant changes in sleep between PBS and TNFα injected flies (* = p < 0.05). (C-F) Student’s t-tests revealed that the increase in sleep was not associated with an increase in bout length during the day or night in either genotype (p > 0.05).