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. Author manuscript; available in PMC: 2018 Oct 31.
Published in final edited form as: Nat Med. 2016 Jul 18;22(8):915–923. doi: 10.1038/nm.4134

Figure 2. Cblb in immune cells protects against fungal pathology.

Figure 2

(a,b) Cblb+/+ mice were reconstituted with bone marrow from Cblb+/+, CblbΔ/Δ, or CblbC373A/C373A mice after lethal irradiation. After full reconstitution mice were infected intravenously with C. albicans (105 CFU/21.5 g body weight) and monitored over time. Plots depict (a) weight loss over time after infection, as compared to starting weight (P values assessed by two-way ANOVA) or (b) survival over time after infection (P values assessed with log rank test). n = 6 for all cohorts. (c,d) Cblb+/+Rag2Δ/Δ or CblbΔ/ΔRag2Δ/Δ mice were infected, scored and analyzed as in (a,b) for (c) weight loss and (d) mortality. n = 7 for Cblb+/+Rag2Δ/Δ, n = 6 for CblbΔ/ΔRag2Δ/Δ mice. (e,f) Rag2Δ/Δ mice were reconstituted with bone marrow from Cblb+/+Rag2Δ/Δ or CblbΔ/ΔRag2Δ/Δ mice after lethal irradiation. Eight weeks after reconstitution mice were infected, scored, and analyzed as in (a,b) for (e) weight loss and (f) mortality. n = 6 for Cblb+/+Rag2Δ/Δ into Rag2Δ/Δ, n = 5 for CblbΔ/ΔRag2Δ/Δ into Rag2Δ/Δ mice. (g,h) Cblb+/+, CblbΔ/Δ, Cblb+/+Rag2Δ/Δ, or CblbΔ/ΔRag2Δ/Δ mice were infected on their shaved dorsum with 2 x 108 CFU C. albicans. Plot depicts fungal load in the skin 3 days after infection, plotted as CFU/total infected skin area. Dots represent individual mice. For panels (a,b,e,f,g,h) 1 representative of 3, for panels (c,d) 1 representative of 4 independent experiments is shown. *P < 0.05, **P < 0.01, ***P < 0.001 as calculated with Student’s t-test, unless stated otherwise.