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. 2018 Oct 31;13(10):e0205256. doi: 10.1371/journal.pone.0205256

Fig 4. Survival of Drosophila melanogaster flies pre-exposed to non-pathogenic bacteria and subsequently injected with pathogenic bacteria.

Fig 4

Emerged flies pre-exposed to Luria-Bertani (LB) broth, a non-pathogenic strain of Escherichia coli (Ec), or heat-killed E. coli (HK Ec) were transferred to fresh untreated diet and then injected with (A) phosphate-buffered saline (PBS), (B) E. coli, (C) Photorhabdus luminescens, or (D) P. asymbiotica. Survival was monitored for 72 hours post injection. Survival experiments were replicated three times and results were analyzed using Log-rank (Mantel-Cox) test in GraphPad Prism software.