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. 2018 Oct 31;13(10):e0205496. doi: 10.1371/journal.pone.0205496

Fig 3. Exosome isolation by the size exclusion chromatography.

Fig 3

In (a) representative immunoblot showing the distribution of exosome markers (CD63, CD9, CD81) and high-abundance serum proteins (illustrated by Ponceau S staining) in the successive SEC fractions of a FaDU culture medium. In (b) total protein concentrations (μg/uL) in the subsequent SEC fractions. In (c) culture medium supplemented with 5% ED FBS and NOT co-cultured with cells was analyzed as in Panel A; “E+” denotes exosome-containing positive control.