Figure 3.

Effect of Gemcitabine, 5-Fluorouracil (5-FU), and 4-Methylumbelliferone (4-MU) on PANC-1 tumor in the 3D co-culture system. PANC-1 cells resuspended in RAD16-I solution were injected into a peptide matrix containing fibroblasts (day 0). Gemcitabine, 5-FU, or 4-MU were added 24 h after the injection. Cells were incubated with the drug for 72 h and assessed for cellular density and viability. Cellular density was evaluated at different time points with phase contrast microscopy, while cellular viability was assessed with Live/Dead at day 4 from the injection. Scale bar: 100 µm.