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Effect of Gemcitabine, 5-FU, and 4-MU on the 3D co-culture system. (A) Toluidine blue staining of 3D co-cultures. Cellular density at day 4 was evaluated using toluidine blue staining. Scale bar: 100 µm. (B) MTS assay. MTS was performed on PANC-1 injected in an empty gel (left) and on co-cultures and hNDF monocultures (right). N = 3, n = 3.
