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. 2018 Sep 13;34(40):12083–12092. doi: 10.1021/acs.langmuir.8b01272

Figure 5.

Figure 5

Fluorescence emission ratio of SYFP2 to mTurquoise2 (527 nm/475 nm) as a function of time following the exchange of proteins between SYFP2-C3Ms and mTurquoise2-C3Ms at different final FRET pair amounts: 50% (yellow solid dots), 40% (green open squares), 30% (cyan open triangles), and 20% (blue open diamonds). The total concentrations of proteins and polymer are 1 and 0.18 μM, respectively. The solid lines are the curves obtained using eq 9, with k = 1.0 × 10–2 s–1 and RFRET,eq = 2.39 for 50%, RFRET,eq = 1.86 for 40%, RFRET,eq = 1.24 for 30%, and RFRET,eq = 0.81 for 20%. Error bars represent the standard deviation (nexp = 4).