Table 1. Final Fluorescence Emission Ratios of SYFP2 to mTurquoise2 (RFRET,eq) after C3M Protein Exchange with a Kinetic Coefficient of (1.0 × 10–2) ± (0.1 × 10–2) s–1a.
FRET pair amount (%) | RFRET,eq |
---|---|
20 | 0.81 ± 0.01 |
30 | 1.24 ± 0.01 |
40 | 1.86 ± 0.02 |
50 | 2.39 ± 0.02 |
Standard deviations are based on multiple measurements (nexp = 4).