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. 2018 Oct 31;13(10):e0206606. doi: 10.1371/journal.pone.0206606

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© 2018 Gajenthra Kumar et al

This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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Fig 3 — Confirmation of PC 38:4 in positive (A) and negative ion mode (B), respectively. Upon fragmentation of the precursor ion of m/z 810.7 in positive ion mode the phosphatidylcholine ion corresponding to PC head group, mz/184.1 is generated [67]. In negative ion mode the corresponding acetate adduct of m/z 868.5, generates the expected fragment ions corresponding to neutral loss as ketenes and acyl anions of m/z 283.3 (FA 18:0) and m/z 303.2 (FA 20:4) respectively. From the precursor ion masses (m/z 868.5) and generated fragment ions (m/z 283.3 and m/z 303.2) the lipid can be confirmed as a PC 18:0–20:4 (PC 38:4 as sum formula)[61].