Fig 7. CNTCOOH NPs interfere with the LDH assay.

HUVECs were treated with 0.5 μM camptothecin, 1 μM staurosporine, 4 mM H₂O₂ or 0.1% Triton X-100 for 24 hours to induce LDH release. After the treatment, the media were collected and centrifuged and the supernatant was divided into two aliquots. One aliquot was mixed with 100 μg/ml of CNTCOOHs and the other was left as a control. The presence of active LDH was measured colorimetrically. Data measured in 4-plicates (n = 3) are presented as the means ± SEM as tested by the two-tailed unpaired t-test, where ***P<0.001 versus the control.