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. 2018 Oct 19;16(10):e3000041. doi: 10.1371/journal.pbio.3000041

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© 2018 Chen et al

This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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Fig 3 — Subcellular localisation of endogenously tagged Par-6–GFP (A) and GFP-aPKC (B), as revealed by anti-GFP staining (green). MARCM clones (marked by GFP, green) homozygous mutant for par-6^Δ226 (C) and aPKC^HC (D, E) show normal apical actin brush borders (F-actin in red) and SJ localisation (E; Mesh, red). The apical localisation of Par-6 (red) is lost in aPKC^K06043 MARCM clones (marked by GFP, green) (F) as is the apical localisation of aPKC (red) in par6^Δ226 MARCM clones (marked by GFP, green) (G). Scale bars, 10 μm. aPKC, atypical protein kinase C; EC, enterocyte; GFP, green fluorescent protein; MARCM, mosaic analysis with a repressible cell marker; SJ, septate junction.